ABSTRACT
Aims: SA (Salicylic acid) is a key regulator for sugar metabolic pathway, but the regulating function on sugar metabolism in tomato seedlings is imperfect. In this study, tomato seedlings were sprayed with SA, and then soluble sugar contents, enzyme related to sugar metabolism levels, gene expressions were determined. Study Design: In order to analyze the mechanism of SA in sugar metabolic pathway, tomato seedlings were sprayed with SA, and then soluble sugar contents, enzyme related to sugar metabolism levels, gene expressions were determined. Place and Duration of Study: College of Biological Science and Technology, between December 2021 and May 2022. Methodology: The expressions of genes related to sugar metabolism in tomato were measured by qRT-PCR. The contents of soluble sugar in tomato seedings were measured with HPLC (High performance liquid chromatography). Results: The sugar metabolism in tomato seedlings was affected by SA treatment for 6 h. The contents of fructose, glucose and sucrose were improved in tomato seedlings by SA treatment. Between 6 to 24 h, enzymatic activities of SlAI, SlNI and SlSS were higher than those in controls, while there was little change in enzyme activity of SISPS. Conclusion: The contents of soluble sugar, enzyme activities related to sugar metabolism had changed considerably by SA treatment. Especially from 6 h to 24 h after SA treatment, the contents of fructose and glucose were significantly higher than those of the control group, and the changes of sugar contents were mainly related to the increases of enzyme activities of AI, NI and SS, suggesting that SA treatment mainly affected the activities of SlAI, SlNI and SlSS to promote the accumulations of soluble sugars.
ABSTRACT
El crecimiento longitudinal de tallos y raíces fue investigado en plántulas de tomate (Solanum lycopersicum L), luego de la aplicación de cuatro aislamientos de Trichoderma asperellum: T25, T46, T84 y T109. Sólo la aplicación del aislamiento T109 causó un incremento significativo (p ≤ 0,05) del crecimiento longitudinal de tallos y raíces. El peso seco de las plántulas también fue estimulado desde la primera semana luego de la aplicación de T109. El crecimiento fue estimulado significativamente (p ≤ 0,05) por la aplicación de 105 y 106 conidias/ml, pero no lo fue por la aplicación de 104, 107 y 108 conidias/ml. Estos resultados indican que el estímulo del crecimiento producido por T. asperellum en plántulas de tomate, sólo ocurre con aislamientos particulares y un rangos de concentración específicos.
Longitudinal growth of shoot and roots was investigated in tomato seedlings (Solanum lycopersicum L) after application of four isolates of T. asperellum (T25, T46, T84 y T109). Only the isolate T109 caused a significant (p ≤ 0.05) increase of longitudinal growth of roots and shoots, and dry weight of seedlings was also stimulated from the first week after T109 application. The growth was significantly (p ≤ 0.05) stimulated by 105 and 106 conidia/ml, but it was not by 104, 107 and 108 conidia/ml. These results indicate that growth stimulus caused by T. asperellum in tomato seedlings, occurs only with specific isolates and at a specific concentration ranges.